C3larvin Toxin, an ADP-ribosyltransferase from Paenibacillus larvae
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چکیده
منابع مشابه
ADP-ribosyltransferase from Hen Liver Nuclei
Chromatin-bound ADP-ribosyltransferase from adult hen liver nuclei was purified to a homogeneous state through salt extraction, gel filtration, hydroxyapatite, phenyl-Sepharose, Cm-cellulose, and DNA-Sepharose. The ADP-ribosyltransferase has a pH optimum at 9.0 and does not require DNA for reaction. The purified enzyme has a molecular weight of 27,500 2 500. Agmatine sulfate, arginine methyl es...
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In addition to the two large clostridial cytotoxins (TcdA and TcdB), some strains of Clostridium difficile also produce an actin-specific ADP-ribosyltransferase, called binary toxin CDT. We used a PCR method and Southern blotting for the detection of genes encoding the enzymatic (CDTa) and binding (CDTb) components of the binary toxin in 369 strains isolated from patients with suspected C. diff...
متن کاملCharacterization of the enzymatic component of the ADP-ribosyltransferase toxin CDTa from Clostridium difficile.
Certain strains of Clostridium difficile produce the ADP-ribosyltransferase CDT, which is a binary actin ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component (CDTa) of the toxin using the binding component of Clostridium perfringens iota toxin (Ib), which...
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An ADP-ribosyltransferase was found in elongation factor 2 (EF-2) preparations from polyoma virus-transformed baby hamster kidney (pyBHK) cells. Like fragment A of diphtheria toxin and Pseudomonas toxin A, this eukaryotic cellular enzyme transfers [14C]adenosine from NAD+ to EF-2. However, the cellular transferase is immunologically distinct from fragment A. The transferase also can be distingu...
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Takai, Y., Kishimoto, A,, Kawahara, Y., Minakuchi, R.. Sano, K., Kikkawa, U., Mori, T., Yu, B., Kaibuchi, K. & Nishizuka, y. (1981) Adv. Cyclic Nucleotide Res. 14, 301-313 Thomas, D. D. & Knoop, F. C. (1982) J. Inject Dis. 145, 141147 Thomas, D. D. & Knoop, F. C. (1983) J . Infect. Dis. 147,450-459 Turnberg, L. A., Bieberdorf, F. A,, Morawski, S. G . & Fordtran, J. S. (1970) J. Clin. Invest. 49...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2015
ISSN: 0021-9258
DOI: 10.1074/jbc.m114.589846